Cytotoxicity effects of lipophilic extracts of Thai herbs in human breast cancer cells and Human Epidermoid Carcinoma cells

Author

Yusuk, Y. ; Gaywee, J. ; Ruang-Areerate, Toon ; Rungsuriyaviboon, O.

Author_Institution

Dept. of Med. Technol., Thammasart Univ., Bangkok, Thailand

fYear

2012

fDate

4-7 Nov. 2012

Firstpage

106

Lastpage

110

Abstract

This study was to evaluate the cytotoxic activity of lipophilic extracts of Thai herbs (Harrisonia perforata, Pongamia Pinnata, Azadirachta indica) against from Human Breast Cancer (MCF-7) cells, Human Epidermoid Carcinoma (KB-31) cells and from Monkey kidney (Vero) cells were tested by MTT assay against Vero, KB-31 and MCF-7 cells line. Apoptotic induction was tested in the MCF-7 and used Vero cells as control was analyzed by electrophoresis. All extracts did not induce cytotoxicity against Vero cells. A. indica leaves showed the highest cytotoxicity effect against MCF-7 cells (IC₅₀ = 296.49 μg/ml) and KB-31 cells (IC₅₀ 199.49 μg/ml), whereas H.perforata stem bark showed the highest cytotoxicity effect against KB-31 cells (IC₅₀= 191.06μ/ml). Leaves of H.perforata and P.pinnata had not produced cytotoxicity against three cell lines.

Keywords

botany; cancer; cellular biophysics; patient treatment; A. indica leaves; Azadirachta indica; Harrisonia perforata; KB-31 cells; MCF-7 cells; MTT assay; Monkey kidney; Pongamia Pinnata; Thai herbs; Vero cells; apoptotic induction; cytotoxic activity; cytotoxicity effects; electrophoresis; human breast cancer cells; human epidermoid carcinoma cells; lipophilic extracts; stem bark; Apoptosis; Cytotoxicity; Human Breast Cancer cells; Human Epidermoid Carcinoma;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Molecular Medicine and Engineering (NANOMED), 2012 IEEE 6th International Conference on

Conference_Location

Bangkok

ISSN

2159-6964

Print_ISBN

978-1-4673-5101-0

Type

conf

DOI

10.1109/NANOMED.2012.6509131

Filename

6509131