Time-resolved Two-photon Excitation Fluorescence Spectroscopy Using a Streak Camera

Author

Liu, Lixin ; Qu, Junle ; Lin, Ziyang ; Guo, Baoping ; Liu, Wenqing ; Niu, Hanben

Author_Institution

Shenzhen Univ., Shenzhen

fYear

2007

fDate

23-27 May 2007

Firstpage

964

Lastpage

968

Abstract

We present a time-resolved two-photon excitation fluorescence spectroscopy system that is based on a picosecond streak camera and a prism spectrophotometer, and can perform simultaneous fluorescence spectrum and lifetime measurement using two-photon excitation. The potential applications of the system in biomedicine have been demonstrated with tissue endogenous fluorophore (flavin adenine dinucleotide), plant specimen (aloe epidermis) and gastric smooth muscle tissue.

Keywords

biomedical optical imaging; fluorescence spectroscopy; muscle; optical prisms; spectrophotometers; streak cameras; time resolved spectroscopy; two-photon processes; two-photon spectroscopy; aloe epidermis; biomedicine; flavin adenine dinucleotide; gastric smooth muscle tissue; plant specimen; prism spectrophotometer; streak camera; time-resolved spectroscopy; tissue endogenous fluorophore; two-photon excitation fluorescence spectroscopy; Biomedical engineering; Biomedical imaging; Biomedical measurements; Cameras; Charge-coupled image sensors; Epidermis; Fluorescence; Microscopy; Muscles; Spectroscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-1077-4

Electronic_ISBN

978-1-4244-1078-1

Type

conf

DOI

10.1109/ICCME.2007.4381883

Filename

4381883