DocumentCode
2091553
Title
Optimization of Condition for Maximum Recovery of Antioxidants from Cephalotaxus sinensis and their FRAP Assay
Author
Saeed, Muhammad Khalid ; Deng, Yulin ; Dai, Rongji ; Yu, Yuhong
Author_Institution
Beijing Inst. of Technol., Beijing
fYear
2007
fDate
23-27 May 2007
Firstpage
1664
Lastpage
1667
Abstract
In this study, Cephalotaxus sinensis (Rehd et Wile) Li (Cephalotaxaeeae), plant was examined for phenolic constituents and antioxidant capacity, to determine their potential as a source of naturat antioxidants. Total polyphenol content (TPC) were determined spectrophotometrically, after extraction of ptant material with assorted solvents at 80degC for 3 hour. Antioxidant activity of aqueous extracts and fractions were evaluated by FRAP assay. The highest antioxidant activity was observed in the extract of 80% aq. ethanol followed by fractionation with 40% (v/v) aq. ethanoi using AB-8 resin, which had soaring leveis of potyphenot. Resutts strongty suggested that potyphenot content shoufd be considered as an imperative feature of Cephalotaxus sinensis ptant and demonstrate a impending for exptoit as natural antioxidants.
Keywords
biochemistry; spectrophotometry; Cephalotaxaeeae; Cephalotaxus sinensis; FRAP assay; antioxidants; spectrophotometry; total polyphenol content; Chemicals; Ethanol; Fractionation; Humans; Methanol; Optimization methods; Organisms; Protection; Resins; Solvents; Cephalotaxus sinensis; FRAP; Optimization; antioxidants;
fLanguage
English
Publisher
ieee
Conference_Titel
Complex Medical Engineering, 2007. CME 2007. IEEE/ICME International Conference on
Conference_Location
Beijing
Print_ISBN
978-1-4244-1077-4
Electronic_ISBN
978-1-4244-1078-1
Type
conf
DOI
10.1109/ICCME.2007.4382030
Filename
4382030
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