A test target for estimating the resolution of a holographic microscope

Author

Kaikkonen, Ville A. ; Mäkynen, Anssi J.

Author_Institution

Centre for Meas. & Inf. Syst., Univ. of Oulu, Kajaani, Finland

fYear

2011

fDate

10-12 May 2011

Firstpage

1

Lastpage

5

Abstract

A method for testing the resolution of a holographic microscope with a set of in-house test targets is presented. The test targets are made of calibrated microparticles mixed in clear polyurethane medium and sealed between two glass plates. The depth range inside the target that can be imaged with adequate lateral resolution with different holographic microscope setups is demonstrated by studying resolvability of the microparticles from the reconstructed hologram images using Sparrow´s definition for the resolution limit. In addition, the effect of different particle concentrations to image signal-to-noise-ratio is evaluated.

Keywords

calibration; holography; image reconstruction; image resolution; optical microscopes; optical testing; calibrated microparticle; clear polyurethane medium; glass plate; hologram image reconstruction; holographic microscope resolution estimation; holographic microscope resolution testing; image signal-to-noise-ratio; in-house test targets; particle concentration effect; resolvability; Detectors; Image reconstruction; Image resolution; Light sources; Microscopy; Pixel; Signal to noise ratio; 3D; Laser; diffraction; resolving power;

fLanguage

English

Publisher

ieee

Conference_Titel

Instrumentation and Measurement Technology Conference (I2MTC), 2011 IEEE

Conference_Location

Binjiang

ISSN

1091-5281

Print_ISBN

978-1-4244-7933-7

Type

conf

DOI

10.1109/IMTC.2011.5944285

Filename

5944285