A sensitive, rapid and specific technique for the detection of antigen-specific cells on shear horizontal surface acoustic wave (SH-SAW) sensors

The current study presents an innovative concept of antigen-specific cells micro-incubator, rapid purification of tumor cells (TCs) on shear horizontal surface acoustic wave (SH-SAW) sensors. With the specific antibodies conjugated onto the surface of the SH-SAW sensors can be used to specifically recognize the TCs in the clinical body fluids. The novel micro-incubator was developed for incubation process in the purification process when a relatively large amount of bio-sample was required. Large amount of clinical bio-samples (>; 100 ml) can be flowed efficiently within a short period of time (less than 60 minutes). First, the mixture of colorectal adenocarcinoma HCT-8 and medium was simulated blood of patients. In addition, successful demonstration for detection of cells has been achieved by performing on-chip identification.