3D reconstruction of 2D crystals from projections in real space

Author

Marabini, Roberto ; Herman, Gabor T. ; Sorzano, Carlos O S ; Carazo, José M. ; Fernández, José J. ; Matej, Samuel

Author_Institution

Graduate Center, City Univ. of New York, NY, USA

fYear

2002

fDate

2002

Firstpage

689

Lastpage

692

Abstract

A new algorithm for 3D reconstruction of two-dimensional crystals from projections is presented, and applied to biological macromolecules imaged using electron microscopy. Its main departure from the traditional approach is that it works in real space, rather than in Fourier space, and is iterative. This approach has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the electron microscope image formation process.

Keywords

biological techniques; biology computing; electron diffraction crystallography; image reconstruction; iterative methods; macromolecules; molecular biophysics; transmission electron microscopy; 2D crystals; 3D reconstruction algorithm; Fourier space; biological macromolecules; biological material; biophysical technique; electron microscope image formation process; more accurate modeling; transmission electron microscopy; Crystallization; Crystals; Electron microscopy; Fourier transforms; Image reconstruction; Iterative algorithms; Iterative methods; Lattices; Molecular biophysics; Transmission electron microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on

Print_ISBN

0-7803-7584-X

Type

conf

DOI

10.1109/ISBI.2002.1029351

Filename

1029351