Image restoration in sets of noisy electron micrographs

Author

Ludike, S.J. ; Chiu, Wah

Author_Institution

Nat. Center for Macromolecular Imaging, Baylor Coll. of Med., Houston, TX, USA

fYear

2002

fDate

2002

Firstpage

745

Lastpage

748

Abstract

The imaging process in a transmission electron microscope (TEM) produces a number of artifacts including the contrast transfer function (CTF) and envelope functions. In addition, when electron cryomicroscopy is performed for purposes of single particle reconstruction, signal to noise ratios are very low, generally peaking between 0.1 and 1.0, and averaging 0.01 to 0.1. We apply a semi-empirical model of the microscope artifacts to optimally correct data used for 3D single particle reconstructions.

Keywords

biological techniques; image reconstruction; image restoration; low-temperature techniques; molecular biophysics; transmission electron microscopy; 3D single particle reconstructions; 3D structure; TEM; artifacts; averaging; biological molecules; contrast transfer function; electron cryomicroscopy; envelope fluctuations; image restoration; imaging process; macromolecular assemblies; noisy electron micrograph sets; semi-empirical model; signal to noise ratios; single particle reconstruction; transmission electron microscope; Biomedical imaging; Coordinate measuring machines; Educational institutions; Energy resolution; Fourier transforms; Image reconstruction; Image restoration; Signal resolution; Signal to noise ratio; Transmission electron microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging, 2002. Proceedings. 2002 IEEE International Symposium on

Print_ISBN

0-7803-7584-X

Type

conf

DOI

10.1109/ISBI.2002.1029365

Filename

1029365