Exploration the load of molecular motors based on F0F1-ATPase

Author

Cunshe, Chen ; Xiaojuan, Li ; Huitian, Lv

Author_Institution

Sch. of Chem. & Environ. Eng., Beijing Technol. & Bus. Univ., Beijing

fYear

2006

fDate

17-20 Dec. 2006

Firstpage

329

Lastpage

333

Abstract

The potential of motor protein F₀F₁-ATPase is explored in the paper. A fluorescence probe F1300 labeled in inner chromatophores was used as a pH indicator for detecting proton flux driven by ATP synthesis in F₀F₁-ATPase. Furthermore, the F₁beta subunit of F₀F₁-ATPase was attached by a system of anti-beta antibody-biotin-avidin-biotin-second antibody (specificfor clenbuterol) as a load of molecular motors. The capturing mechanism was based on antibody-antigen reaction, while the detecting mechanism depended on fluorescence change responding to pH changes during rotary catalytic A TP synthesis process. The results shows that the activity of F₀F₁-ATPase-ATPase is affected with different loads.

Keywords

biomechanics; fluorescence; molecular biophysics; proteins; ATP synthesis; F₀F₁-ATPase; antibody-antigen reaction; chromatophore; fluorescence probe F1300; molecular motor; motor protein; pH indicator; proton flux; Biochemistry; Biomimetics; Chemical technology; Fluorescence; Micromotors; Probes; Protons; Rabbits; Robots; Sugar; F0F1-ATPase; clenbuterol; fluorescence probe; molecular motors; proton flux;

fLanguage

English

Publisher

ieee

Conference_Titel

Robotics and Biomimetics, 2006. ROBIO '06. IEEE International Conference on

Conference_Location

Kunming

Print_ISBN

1-4244-0570-X

Electronic_ISBN

1-4244-0571-8

Type

conf

DOI

10.1109/ROBIO.2006.340197

Filename

4141886