Monitoring Cerebral Hemodynamics Following Optogenetic Stimulation via Optical Coherence Tomography

Author

Atry, Farid ; Frye, Seth ; Richner, Thomas J. ; Brodnick, Sarah K. ; Soehartono, Alana ; Williams, Julia ; Pashaie, Ramin

Author_Institution

Electr. Eng. & Comput. Sci. Dept., Univ. of Wisconsin-Milwaukee, Milwaukee, WI, USA

Volume

62

Issue

2

fYear

2015

fDate

Feb. 2015

Firstpage

766

Lastpage

773

Abstract

In this article, spectral domain optical coherence tomography is used to measure the hemodynamic response induced by optogenetic stimulation in the somatosensory cortex of transgenic mice. By analyzing the 3-D angiograms and Doppler measurements produced by coherence tomography, we observed significant increase in blood flow as a result of increased vessel diameter and blood velocity following optical stimulation of cortical neurons. Such distinct responses were not observed in control experiments where the brain of wild-type mice were exposed to the same light pulses.

Keywords

Doppler measurement; biomedical optical imaging; blood vessels; brain; cellular biophysics; genetics; haemodynamics; optical tomography; patient monitoring; radiation therapy; somatosensory phenomena; 3D angiogram analysis; Doppler measurements; brain; cerebral hemodynamic monitoring; cortical neurons; optogenetic stimulation; somatosensory cortex; spectral domain optical coherence tomography; transgenic mice; vessel diameter; Biomedical optical imaging; Blood; Laser beams; Mice; Optical imaging; Stimulated emission; Functional monitoring and imaging; Laser Doppler velocimetry.; Medical and biological imaging; Optical coherence tomography; Optogenetics; laser Doppler velocimetry; medical and biological imaging; optical coherence tomography; optogenetics;

fLanguage

English

Journal_Title

Biomedical Engineering, IEEE Transactions on

Publisher

ieee

ISSN

0018-9294

Type

jour

DOI

10.1109/TBME.2014.2364816

Filename

6942241