Pulsed THz protein spectroscopy in organic solvents

Author

Mickan, S.P. ; Dordick, J.S. ; Munch, J. ; Abbott, D. ; Zhang, X.-C.

fYear

2002

fDate

24-24 May 2002

Abstract

Summary form only given. We are interested in using pulsed THz spectroscopy to characterize the conformation and hydration of enzymatic proteins for biochip analysis. We present measurements of the refractive index and extinction coefficient of organic solvents commonly used in studying protein activity. Our spectroscopic instrument is a pulsed THz system, based on a 100-fs Ti:sapphire laser, surface current generation and electro-optic detection. Preliminary results for iso-octane, toluene and cyclohexane show that the protein refractive index is profoundly affected by even small amounts of water present in the solvents. We present a detailed study of the hydration of protein in the non-polar hydrophilic solvent 1,4-dioxane.

Keywords

biological techniques; extinction coefficients; infrared spectroscopy; molecular configurations; organic compounds; proteins; refractive index; solvation; solvent effects; 0.2 to 2 THz; 1,4-dioxane; 100 fs; 100-fs Ti:sapphire laser; biochip analysis; cyclohexane; electro-optic detection; enzymatic protein conformation; enzymatic protein hydration; extinction coefficient; iso-octane; nonpolar hydrophilic solvent; organic solvents; protein activity; pulsed THz protein spectroscopy; refractive index; surface current generation; toluene; Extinction coefficients; Optical pulse generation; Optical refraction; Optical variables control; Physics; Protein engineering; Refractive index; Solvents; Spectroscopy; Vents;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics, 2002. CLEO '02. Technical Digest. Summaries of Papers Presented at the

Conference_Location

Long Beach, CA, USA

Print_ISBN

1-55752-706-7

Type

conf

DOI

10.1109/CLEO.2002.1034422

Filename

1034422