Microelectrothermofluidic packaging for single chip integrated viral RNA extraction and RT-PCR microdevices

This paper presents a microelectrothermofluidic packaging of integrated microdevice for viral ribonucleic acid (RNA) extraction from blood samples and its amplification through a reverse transcription (RT)-polymerase chain reaction (PCR) method for application to the point-of-care (POC) infectious disease diagnostics. Integrated microdevice consists of solid phase extraction microchannel structure, microfluidic mixer, and meander-shaped microchamber for RT-PCR. Metallic microheater and resistive temperature detection (RTD) microsenosr is monolithically integrated with fluidic components. In order to secure both electrical and fluidic interconnections, spring-loaded electrical pin and drilled microchannel structure has been design in polycarbonate micropackaging structure. In the biotesting, we have demonstrated to detect dengue serotype III virus from the 80μl of blood sample with 80pfu virus concentration.