Multiphoton autofluorescence microscopy and second harmonic generation microscopy of oral epithelial neoplasms

Author

Vargas, Gracie ; Shilagard, Tuya ; Ho, Ki-Hong ; McCammon, Susan

Author_Institution

Univ. of Texas Med. Branch, Galveston, TX, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

6311

Lastpage

6313

Abstract

The objective of this study was to evaluate the nonlinear optical microscopy techniques of multiphoton autofluorescence microscopy (MPAM) and second harmonic generation microscopy (SHGM) for noninvasive imaging of oral epithelial carcinogenesis. In vivo imaging was performed in a hamster model for oral carcinogenesis to characterize optical and morphometric alterations during neoplastic transformation. Data is presented showing alterations in morphometry and collagen density during the precancerous phase of neoplastic transformation.

Keywords

bio-optics; biomedical optical imaging; cancer; fluorescence; multiphoton processes; optical harmonic generation; optical microscopy; proteins; tumours; collagen density; morphometric alterations; morphometry; multiphoton autofluorescence microscopy; neoplastic transformation; noninvasive imaging; nonlinear optical microscopy technique; optical alterations; oral epithelial carcinogenesis; oral epithelial neoplasms; second harmonic generation microscopy; Animals; Cricetinae; Equipment Design; Equipment Failure Analysis; Mesocricetus; Mouth Neoplasms; Neoplasms, Glandular and Epithelial; Reproducibility of Results; Sensitivity and Specificity;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5332783

Filename

5332783