Imaging multi-spectral fluorescent probes in living cells at video rates

Author

Delaville, Fernando J. ; Fogarty, Kevin E. ; Fay, Fredric S.

Author_Institution

Dept. of Physiol, Massachusetts Univ. Med. Center, Worcester, MA, USA

fYear

1989

fDate

27-28 Mar 1989

Firstpage

243

Lastpage

244

Abstract

A fast digital imaging fluorescence microscope capable of obtaining multispectral images at video rates (30/s) has been developed. It integrates commercially available equipment with custom-made circuitry and software to record freeze-frame images of 100-ms duration every 1/30 s. The components involved and the sources of noise have been fully characterized to use their models in offline processing. From the raw images, spatial changes in concentration or distribution can be studied as a function of time. The system will be tested with calcium images obtained by loading single isolated smooth muscle cells with Fura-2 fluorescent dye. The system is capable of generating a movie in which the calcium distribution can be seen changing with time

Keywords

biological techniques and instruments; cellular biophysics; fluorescence; Ca distribution; Fura-2 fluorescent dye; commercially available equipment; custom-made circuitry; fast digital imaging fluorescence microscope; freeze-frame images; living cells; offline processing; single isolated smooth muscle cells; video rates; Calcium; Circuit noise; Digital images; Fluorescence; Microscopy; Motion pictures; Multispectral imaging; Muscles; Probes; System testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference, 1989., Proceedings of the 1989 Fifteenth Annual Northeast

Conference_Location

Boston, MA

Type

conf

DOI

10.1109/NEBC.1989.36791

Filename

36791