DocumentCode
2386236
Title
Widefield fluorescence sectioning with HiLo microscopy
Author
Mertz, Jerome ; Lim, Daryl ; Chu, Kengyeh K. ; Bozinovic, Nenad ; Ford, Timothy
Author_Institution
Dept. of Biomed. Eng., Boston Univ., Boston, MA, USA
fYear
2009
fDate
3-6 Sept. 2009
Firstpage
3229
Lastpage
3230
Abstract
HiLo microscopy is a widefield fluorescence imaging technique that provides depth discrimination by combining two images, one with non-uniform illumination and one with uniform illumination. We discuss the theory of this technique and a variety of practical implementations in brain-tissue imaging and fluorescence endomicroscopy.
Keywords
biomedical optical imaging; brain; fluorescence; optical microscopy; HiLo microscopy; brain-tissue imaging; depth discrimination; fluorescence endomicroscopy; nonuniform illumination; uniform illumination; widefield fluorescence sectioning; Animals; Equipment Design; Humans; Image Processing, Computer-Assisted; Microscopy, Confocal; Microscopy, Fluorescence; Optics and Photonics; Reproducibility of Results;
fLanguage
English
Publisher
ieee
Conference_Titel
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location
Minneapolis, MN
ISSN
1557-170X
Print_ISBN
978-1-4244-3296-7
Electronic_ISBN
1557-170X
Type
conf
DOI
10.1109/IEMBS.2009.5333166
Filename
5333166
Link To Document