Detecting alterations in cell ultrastructure with optical imaging

Author

Backman, Vadim ; Subramanian, Hariharan ; Pradhan, Prabhakar ; Liu, Yang ; Capoglu, Ilker ; Rogers, Jeremy D. ; Roy, Hemant K. ; Taflove, Allen

Author_Institution

Northwestern Univ., Evanston, IL, USA

fYear

2009

fDate

3-6 Sept. 2009

Firstpage

6325

Lastpage

6326

Abstract

Understanding cell functioning at the nanoscale has been hampered in part by the diffraction limited resolution of optical microscopy. We developed partial wave spectroscopic (PWS) microscopy that is capable of quantifying statistical properties of cell structure at the nanoscale. Our animal and human studies demonstrated that alterations in the nanoscale cell architecture is one of the earliest events in carcinogenesis and precedes any other known morphological changes at larger length scales (i.e. microarchitecture).

Keywords

biomedical optical imaging; cancer; cellular biophysics; optical microscopy; statistical analysis; tumours; carcinogenesis; cell structure statistical properties; cell ultrastructure alteration detection; nanoscale cell architecture; optical imaging; partial wave spectroscopic microscopy; Animals; Cell Transformation, Neoplastic; Image Enhancement; Intestinal Neoplasms; Intestines; Mice; Refractometry; Reproducibility of Results; Sensitivity and Specificity;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE

Conference_Location

Minneapolis, MN

ISSN

1557-170X

Print_ISBN

978-1-4244-3296-7

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2009.5333173

Filename

5333173