Construction of Biodegradable Polymer Scaffold by Photolithography

In this study, we succeeded in fine construction of the PLCL (poly-L-lactide-cocaprolactone) scaffold with micrometer range resolution using photolithography. PLCL is a biodegradable polymer which is used as a material of the scaffold to reproduce the blood vessel. Photolithography known as a technique of microfabrication was used for the processing of PLCL. By this technique, we fabricated a sheet-like PLCL scaffold which is patterned straight line ditches. Laminating the sheet-like scaffold, we fabricated microchannels in the PLCL scaffold. By the attachment of HUVECs in the channel, biocompatibility of the sheet-like PLCL scaffold was confirmed. It is expected to evaluate the behavior of cells in the scaffold.