Sample Clean-up of Red Fluorescent Protein for Analysis Using Electric Field in Microfluidic Device

Microfluidic technology allows the design and operation of effective and simple devices for sample preparation and cleanup. Majority of current sample preparation methods is focused on the complex and combined steps such as solid phase extraction and membrane dialysis. In addition, it is generally recognized that sample treatment often is the bottleneck for the rapid analysis of protein due to performing off-chip in many cases. In this study, for an effective cleaning of protein from a urea-rich protein sample, electro-microfluidic desalting system was applied using the charge characteristics of protein.