Interdigitated electrodes based on impedance biosensor for sensing peptide LL-37

Author

Lee, Jia-Yi ; Wang, Chung-Yih ; Huang, Chi-Fang ; Cheng, An-Ting

Author_Institution

Institute of Electrical Engineering, Tatung University, 40, Chung-Shan N. Rd., Sec. 3, Taipei 104, Taiwan

fYear

2011

fDate

Aug. 30 2011-Sept. 3 2011

Firstpage

71

Lastpage

74

Abstract

An antimicrobial peptide, LL-37, is found in an innate defense system of humans. Patients who suffer urinary tract infection (UTI) will generate LL-37 and which is released into urine. LL-37 can be used as an indicator for the diagnosis of UTI. We have designed a biosensor with an interdigitated electrode on a printed-circuit board (PCB). The surface of the electrode was modified with 3-mercaptopropionic acid and immobilized with anti-LL37 antibody to improve the specificity of the biosensor. By de-embedding jig impedance, the impedance associated with the change of LL-37 concentration was calculated. The sensitivity of this biosensor for LL-37 in a urine sample can reach 50 μg/mL.

Keywords

Biosensors; Capacitance; Electrodes; Impedance; Molecular biophysics; Peptides; Surface impedance; Biosensor; LL-37; impedance; interdigitated electrode; urinary tract infection; Antimicrobial Cationic Peptides; Biosensing Techniques; Conductometry; Electric Impedance; Electrodes; Equipment Design; Equipment Failure Analysis; Humans; Immunoassay; Reproducibility of Results; Sensitivity and Specificity;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE

Conference_Location

Boston, MA

ISSN

1557-170X

Print_ISBN

978-1-4244-4121-1

Electronic_ISBN

1557-170X

Type

conf

DOI

10.1109/IEMBS.2011.6089899

Filename

6089899