Clinical system for skin tumour detection by fluorescence lifetime imaging

Author

Cubeddu, R. ; Comelli, D. ; Andrea, C.D. ; Taroni, P. ; Valentini, G.

Author_Institution

Dipt. di Fisica, Politecnico di Milano, Milan, Italy

Volume

3

fYear

2002

fDate

23-26 Oct. 2002

Firstpage

2295

Abstract

A portable system based on Fluorescence Lifetime Imaging has been developed and tested for the detection of skin tumors in humans. The Heme precursor δ-aminolevulinic acid, which promotes the preferential accumulation of the endogenous Protoporphyrin IX in proliferative tissues, is used as an exogenous marker to target the tumor. Then, using a gated intensified camera, two or more images of the sample are acquired after different delays with respect to the excitation pulses. The images are processed in order to calculate the spatial map of the fluorescence decay time of the sample. The localization of the tumor is based on the longer decay time detected in neoplastic tissues with respect to healthy ones.

Keywords

biochemistry; biomedical equipment; biomedical optical imaging; fluorescence; skin; tumours; basal cell carcinoma; clinical system; diagnostic procedures; endogenous Protoporphyrin IX; excitation pulses; fluorescence decay time spatial map; fluorescence lifetime imaging; gated intensified camera; healthy tissues; heme precursor δ-aminolevulinic acid; medical diagnostic imaging; neoplastic tissues; preferential accumulation; proliferative tissues; skin tumour detection; Cancer; Fiber lasers; Fluorescence; Laser excitation; Lesions; Life testing; Pump lasers; Skin neoplasms; System testing; Tumors;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1053289

Filename

1053289