Effects of Enhanced UV-B Radiation on the Cell Mitosis of the Callus in Wheat

In order to study effects of enhanced ultraviolet-B (280 nm-320 nm) radiation on the cell mitosis, mature embryos of wheat as explant were cultured in the Murashige and Skoog (MS) medium with different concentration of 2, 4-D and 6-BA. The results indicated the inducing rate of the mature embryos from wheat on the MS with 2, 4-D 1.0 mg/L was higher and the callus was better than on the other culture medium. The chromosomal mutation and mitosis of the callus in wheat were studied under the condition of the enhanced UV-B radiation. The results showed that enhanced UV-B radiation inhibited the cell division of callus in wheat, several types of chromosomal aberration such as micronucleus, multi-nucleus, nuclear aberration occurred during the interphase, which may be the results of UV-B inhabiting the DNA replication of the cell to lead to the cell DNA mutation and form the pyrimidine dimer.