Enzymatic Pretreatment and Microwave Extraction of Asiaticoside from Centella asiatica

The extraction of asiaticoside from Centella asiatica by enzymatic pretreatment and microwave extraction (EPME) was studied in this article. The effects of several important factors such as temperature of enzymatic pretreatment, liquid to solid ratio and microwave radiation time were investigated by orthogonal central composite design experiment. An extraction model with well forecast performance was established. The results indicate that the optimum extraction condition was as follows: liquid to solid ratio was 36 mL/g, temperature of enzymatic pretreatment was 45degC, enzymatic time was 30 min, and microwave radiation time was 110 s. On such conditions the yield was 27.10%.