RELIABLE MOTION DETECTION AND ANALYSIS IN LIVE-CELL IMAGING

We describe an alternative method to tracking for analyzing the motion of fluorescent spots in 3D+time video-microscopy. First, we realize motion detection by viewing the 3D+T spatio-temporal as a single 4D volume and detecting simultaneous significant change in the fluorescence of groups of neighboring voxels as spatio-temporal locations with high intensity. This step outputs spatio-temporal locations of motion and are next used to detect the moving spots and making statistical analysis of their motion. We show in particular that we are able to estimate the histogram of the motion speed using only the so segmented spatio-temporal locations. Simulated sequences are used to learn some of the parameters of this second step of our method. Results obtained on simulated sequences as well as on video-microscopic sequences are shown