Study on Loop-Mediated Isothermal Amplification Assay for Detection of Salmonella in Meat Products

Author

Wang Yu ; Zhang Wei ; Yuan Yaowu ; Ma Xiaoyan ; Zhang Huiyan ; Su Xudong ; Wang Zhenqiang ; Ma Bo

Author_Institution

Coll. of Food Sci. & Technol., Agric. Univ. of Hebei, Baoding, China

fYear

2009

fDate

11-13 June 2009

Firstpage

1

Lastpage

6

Abstract

A novel nucleic acid amplification method, termed Loop-mediated Isothermal Amplification (LAMP), which amplifies DNA with high specificity, efficiency, andrapidity under isothermal conditions using a set of four specially designed primers and a DNA polymerase with strand displacement activity, may be a valuable tool for the rapid detection of infectious diseases. This study designed two pairs of gene-specific primers of conservative invA gene, this study establish the LAMP assay of detection of Salmonella successfully, and detection of Salmonella in meat products by LAMP, which is high specifical and sensitive, also save time for the detection of pathogens in food. So it provides the technology platform and technical support for the general detection organizations to promote this technology.

Keywords

DNA; biochemistry; biological techniques; cellular biophysics; diseases; enzymes; food products; food safety; genetics; molecular biophysics; DNA polymerase; Salmonella detection; andrapidity; food safety; gene-specific primers; infectious diseases; invA gene; loop-mediated isothermal amplification assay; meat products; nucleic acid amplification method; pathogen detection; strand displacement activity; DNA; Diseases; Food technology; Humans; Isothermal processes; Lamps; Microorganisms; Pathogens; Polymers; Sequences;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4244-2901-1

Electronic_ISBN

978-1-4244-2902-8

Type

conf

DOI

10.1109/ICBBE.2009.5163482

Filename

5163482