Motor protein motion along microtubules for molecular detection

Author

Tarhan, M.C. ; Yokokawa, R. ; Qiu, H. ; Karsten, S.L. ; Fujita, H.

Author_Institution

CIRMM, Univ. of Tokyo, Tokyo, Japan

fYear

2011

fDate

5-9 June 2011

Firstpage

886

Lastpage

889

Abstract

A molecular detection system was realized using the change in the characteristics of the kinesin-coated bead motion along protein-attached microtubules. A hybrid bio/microfluidics device with a microtubule immobilized main channel and protein-injected sub-channels allowed us to compare different protein attachments and several control experiments with the same experimental conditions. As kinesin/microtubule system is an essential component of neurons, the proposed device is a promising tool for diagnostics of several neurodegenerative diseases.

Keywords

bioMEMS; diseases; microfluidics; molecular biophysics; neurophysiology; patient diagnosis; proteins; hybrid biomicrofluidics device; kinesin coated bead motion; kinesin-microtubule system; microtubule immobilized main channel; microtubule motor protein motion; molecular detection system; neurodegenerative disease diagnostics; protein attached microtubules; protein injected subchannels; Alzheimer´s disease; Bars; Microfluidics; Protein engineering; Proteins; Velocity measurement; Kinesin; Microtubule; Molecular detection; Tau;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969363

Filename

5969363