DocumentCode
2533152
Title
Real-time kinetics measurements of protein induced conformational changes in DNA
Author
Spuhler, Philipp S. ; Knezevic, Jelena ; Yalcin, Ayca ; Dröge, Peter ; Rant, Ulrich ; Ünlü, M. Selim
Author_Institution
Biomed. Eng., Boston Univ., Boston, MA, USA
fYear
2009
fDate
4-8 Oct. 2009
Firstpage
545
Lastpage
546
Abstract
We combine electrical orientation of surface bound dsDNA probes with an optical technique for kinetic measurement of protein-induced conformational changes in DNA. The effects of probe surface density and protein variation are investigated.
Keywords
DNA; molecular biophysics; molecular configurations; proteins; DNA conformational change; electrical orientation; probe surface density; protein; realtime kinetics measurements; Band pass filters; Biomedical measurements; DNA; Electrodes; Fluorescence; Gold; Kinetic theory; Probes; Proteins; Sequences;
fLanguage
English
Publisher
ieee
Conference_Titel
LEOS Annual Meeting Conference Proceedings, 2009. LEOS '09. IEEE
Conference_Location
Belek-Antalya
ISSN
1092-8081
Print_ISBN
978-1-4244-3680-4
Electronic_ISBN
1092-8081
Type
conf
DOI
10.1109/LEOS.2009.5343144
Filename
5343144
Link To Document