Microeddy design and application for single cell trapping and monitoring

Microeddies formed by steady streaming in an oscillating flow can be useful for trapping single-cells in isolation or as arrayed elements. Here, we microfabricate a series of different steady streaming geometries to explore the trapping traits as a function of geometry, placement near or far from walls, and array configuration. To demonstrate device performance, we trap algae and perform single cell assays, with the goal of identifying useful phenotypes for bioenergy applications.