Cortical blood flow imaging with a portable MEMS based 2-photon fluorescence microendoscope

Author

Piyawattanametha, W. ; Solgaard, O. ; Schnitzer, M.J.

Author_Institution

NECTEC, Pathumthani, Thailand

fYear

2011

fDate

5-9 June 2011

Firstpage

1809

Lastpage

1812

Abstract

We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm³) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a PBF for laser excitation and large core fiber for fluorescence collection. We demonstrated cortical blood flow imaging in live mice with transverse (Δx) and axial resolutions (Δz) of 1.6 μm and 13.5 μm, respectively.

Keywords

biomedical optical imaging; endoscopes; fluorescence; haemodynamics; 2-photon fluorescence microendoscope; compound gradient refractive index microlens; cortical blood flow imaging; microelectromechanical systems scanner; photonics bandgap fiber; portable MEMS; Fluorescence; Image resolution; Laser beams; Lenses; Micromechanical devices; Microscopy; 2-photon; MEMS; fluorescence; neuroscience; scanner;

fLanguage

English

Publisher

ieee

Conference_Titel

Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International

Conference_Location

Beijing

ISSN

Pending

Print_ISBN

978-1-4577-0157-3

Type

conf

DOI

10.1109/TRANSDUCERS.2011.5969746

Filename

5969746