Development of protein chips based on self-assembled monolayer and protein A

Author

Jang, Ling-Sheng ; Keng, Hao-Kai ; Hsu, Yi-Chu ; Meldrum, Deirdre R.

Author_Institution

Dept. of Electr. Eng., Nat. Cheng Kung Univ.

fYear

2006

fDate

21-24 May 2006

Lastpage

4358

Abstract

In order to improve the steric hindrances and less ordered structures in the long-chain SAMs, the SAM of 4,4-Dithiodibutyric acid (4,4- DTBA) was investigated to be a thin monolayer for the protein chip. This work presents the feasibility study of 4,4- DTBA as a monolayer of the protein chip based on gold surface. Characterizations of surface topography using atomic force microscopy (AFM), contact angle and fluorescence using protein A-FITC were performed to investigate the binding efficiency for the SAMs of 4,4- DTBA and 11- mercaptoundecanoic acid (11- MUA). The results show that the binding efficiency of 4,4- DTBA is higher than that of 11-MUA. The SAM of 4,4-DTBA can be used as a monolayer for the protein chip and may have a comparable binding efficiency

Keywords

atomic force microscopy; contact angle; fluorescence; monolayers; proteins; self-assembly; surface topography; system-on-chip; 4,4-Dithiodibutyric acid; atomic force microscopy; contact angle; fluorescence analysis; gold surface; less ordered structures; protein A; protein chips; self-assembled monolayer; steric hindrances; surface topography; thin monolayer; Atomic force microscopy; Diseases; Fabrication; Gold; Immune system; Mechanical engineering; Mechanical factors; Protein engineering; Solids; Surface topography;

fLanguage

English

Publisher

ieee

Conference_Titel

Circuits and Systems, 2006. ISCAS 2006. Proceedings. 2006 IEEE International Symposium on

Conference_Location

Island of Kos

Print_ISBN

0-7803-9389-9

Type

conf

DOI

10.1109/ISCAS.2006.1693593

Filename

1693593