Chemical IC chip for dynamical control of protein synthesis

Author

Ikuta, Koji ; Maruo, Shoji ; Fujisawa, Tetsuya ; Fukaya, Yuichiro

Author_Institution

Sch. of Eng., Nagoya Univ., Japan

fYear

1998

fDate

25-28 Nov 1998

Firstpage

249

Lastpage

254

Abstract

The several types of biochemical IC chips with photodetective micro reactors have been developed. The real-time monitoring and control of cell free synthesis of protein was demonstrated successfully. The photo protein of the firefly, the so called Luciferase, was synthesized. The biochemical luminescence was monitored by a built-in photodiode in the micro reactor to transfer reaction status into a voltage signal. Feedback control synthetic reaction was verified experimentally. The results achieved here should be key technology for future DNA and RNA synthesis without a biological cell

Keywords

biochemistry; biocontrol; biological specimen preparation; bioluminescence; biotechnology; feedback; microactuators; microfluidics; micropumps; molecular biophysics; monolithic integrated circuits; photodetectors; process monitoring; proteins; DNA synthesis; Luciferase synthesis; RNA synthesis; biochemical IC chip; biochemical luminescence monitoring; built-in photodiode; dynamic control; feedback control; firefly; photo protein; photodetective micro reactor; protein synthesis; reaction status; voltage signal; Chemicals; Feedback control; Inductors; Integrated circuit synthesis; Luminescence; Monitoring; Photodiodes; Proteins; Signal synthesis; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Micromechatronics and Human Science, 1998. MHS '98. Proceedings of the 1998 International Symposium on

Conference_Location

Nagoya

Print_ISBN

0-7803-5130-4

Type

conf

DOI

10.1109/MHS.1998.745792

Filename

745792