XRPD quantitative analysis of biomaterials based on calcium phosphate

Synthetic calcium phosphate bioceramics are biocompatible materials capable of providing skeleton and scaffolding for new bone growth as well as osteogenesis. Since biological response to the different calcium phosphates changes greatly, quantitative phase analysis is essential to control the properties of the synthetic bioceramics and optimize the biological response. A method of X-ray powder diffraction (XRPD) quantitative analysis has been developed to determine the amounts of the principal crystalline phases (HAP, β-TCP, α-TCP, DCP) in biomaterials based on calcium phosphate. Rutile was selected as the reference, and the Reference Intensity Ratios (RIR) were accurately measured. A RIR database of XRPD quantitative analysis for biomaterials based on calcium phosphate was established. The principals and method of RIR quantitative analysis were introduced. The phase amounts of biomaterials based on calcium phosphate could be analyzed accurately, quickly and conveniently. The relative error is less than 5%. The absorption effect was totally eliminated and the cumbersome calibration curve procedure was shunted. Two samples of calcium phosphate ceramics were analyzed as examples of applicability of the RIR quantitative analysis of biomaterials based on calcium phosphate