A protein structure prediction and function identification

Author

Mao, Rui ; Wu, Kuan

Author_Institution

Coll. of Inf. Eng., Northwest A&F Univ., Yangling, China

fYear

2010

fDate

16-18 April 2010

Firstpage

216

Lastpage

220

Abstract

To study the function of CNV p20 in planta, its bioinformation was analyzed using the software online. The CNV p20 protein sequence was aligned with TBSV p19, a strong RNA silencing suppressor (RSS). The secondary and crystal structure analysis of CNV p20 predicted that it was a RSS based on sequence similar with protein of known function. According to the result of the molecular biology experiments, CNV p20 was required for suppression of GFP transgene silencing. The function of CNV p20 was successfully predicted with its bioinformation and identified with the molecular biology analysis.

Keywords

bioinformatics; crystal structure; molecular biophysics; molecular configurations; proteins; CNV p20 crystal structure analysis; CNV p20 protein sequence; CNV p20 secondary structure analysis; GFP transgene silencing suppression; RNA silencing suppressor; TBSV p19; bioinformation; protein function identification; protein structure prediction; Amino acids; Bioinformatics; Biological information theory; Educational institutions; Genomics; Information analysis; Information science; Protein engineering; Protein sequence; RNA; RNA silencing suppressor; function identification; protein structure; sequence alignment;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on

Conference_Location

Chengdu

Print_ISBN

978-1-4244-6775-4

Type

conf

DOI

10.1109/ICBBT.2010.5478974

Filename

5478974