Stitching terabyte-sized 3D images acquired in Confocal Ultramicroscopy

Author

Bria, Alessandro ; Silvestri, L. ; Sacconi, L. ; Pavone, F. ; Iannello, G.

Author_Institution

Dept. of Autom., Electromagn., Inf. Eng. & Ind. Math., Univ. of Cassino, Cassino, Italy

fYear

2012

fDate

2-5 May 2012

Firstpage

1659

Lastpage

1662

Abstract

A fully automated 3D Stitching tool designed for the very the large images produced by a special class of microscopes capable to acquire volumes of about 1 cm³ at micrometric resolution is presented. Exploiting some unique characteristics of the acquired images the tool makes it possible the accurate reconstruction of images of 1 TB or more in a reasonable time, even on workstations with limited resources. Performance figures confirming the effectiveness of the proposed approach are reported.

Keywords

biomedical optical imaging; image reconstruction; image resolution; medical image processing; optical tomography; confocal ultramicroscopy; fully automated 3D stitching tool; image reconstruction; micrometric resolution; optical tomography; stitching terabyte-sized 3D images; Displacement measurement; Image resolution; Measurement uncertainty; Memory management; Microscopy; Reliability; Tiles; 3D Stitching; Brain Imaging; Confocal Ultramicroscopy; Image Registration; Large Scale Microscopy;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on

Conference_Location

Barcelona

ISSN

1945-7928

Print_ISBN

978-1-4577-1857-1

Type

conf

DOI

10.1109/ISBI.2012.6235896

Filename

6235896