Marching Cube Based Marker-Controlled Watershed Segmentation for CryoEM Density Map

Cryo-electron microscopy (CryoEM) is a very important method for studying the structures of macromolecules. Segmentation is one of the key problems in CryoEM technique. We propose a new 3D watershed, where marching cube is employed as a marker method to control the segmentation. It can transform the domain knowledge into watershed by an interactive interface with isosurface, by which we avoid over-segmentation and obtain the correct segmentation results. Lastly we illustrate the efficiency of the algorithm by testing it on two typical CryoEM density maps.