Integration of an ISFET with a porous microelectrode for application to protein characterization

This paper describes a way of dynamic buffer capacity measurement to characterize proteins, using a coulometric sensor-actuator system. The sensor-actuator is made by integrating an ISFET (ion-sensitive field effect transistor) with a porous microelectrode. Lysozyme and ribonuclease were studied. The results show that the buffer capacity of proteins is both a function of pH and their concentrations. The characters of studied proteins can be distinguished each other from measured curves