Design and fabrication of cubic eggshell containing chick embryo for a novel biomedical platform

We propose a novel biomedical platform of a cubic (artificial) eggshell containing a chick embryo. The use of this proposed platform can eliminate the use of large animals as experimental models, making it more ethically accepted. It also has the advantages of low cost and a small size. The specific design and fabrication method of the cubic eggshell uses polydimethylsiloxane (PDMS) and polycarbonate, which were shown to achieve the required specifications of the proposed platform. The viability of the chick embryo in the cubic eggshell was confirmed through basic experiments. Finally, surgical tools were inserted into the cubic shell and the internal organs were operated on to confirm the use of the developed cubic shell as an appropriate platform for microsurgical training.