Evaluation of stabilizing effect for several monoclonalantibody immobilized quartz crystal microbalance by stabilizer reagents

Author

Park, Jong-Won ; Kurosawa, Shigeru ; Aizawa, Hidenobu ; Naganawa, Ryuichi ; Yamada, Satoshi ; Ishihara, Kazuhiko

Author_Institution

Nat. Inst. of Adv. Ind. Sci. & Technol., Japan

fYear

2003

fDate

4-8 May 2003

Firstpage

978

Lastpage

980

Abstract

We tested five stabilizers for remaining immunologic activity of anti-dinitrophenol (DNP), anti-C-reactive protein (CRP), and anti-2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) antibody immobilized QCM under several storage conditions. Investigated nine stabilizers were as following; 0.25% BlockAce^® and StabilGuard^® as commercial available reagents, 0.2% glycerin and 0.2% bovine serum albumin (BSA) as conventional stabilizers, 0.2% MPC copolymer stabilizer as developed reagent, and PBS solution as blank reagent. According to the experimental results, we found MPC copolymer (NOF Corp., Japan) coated QCM showed highly immunologic activity through specific antigens and their antibodies after the heat acceleration test and long-term storage.

Keywords

biosensors; crystal resonators; microbalances; polymer blends; proteins; MPC copolymer stabilizer; QCM; anti 2,3,7,8 tetrachlorodibenzo-p-dioxin; antiC-reactive protein; antidinitrophenol; antigen; bovine serum albumin; glycerin; heat acceleration test; immunologic activity; monoclonalantibody immobilized quartz crystal microbalance; stabilizer reagent; Biochemical analysis; Bovine; Chemicals; Immune system; Magnetic materials; Photonic crystals; Polymers; Proteins; Temperature; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Frequency Control Symposium and PDA Exhibition Jointly with the 17th European Frequency and Time Forum, 2003. Proceedings of the 2003 IEEE International

ISSN

1075-6787

Print_ISBN

0-7803-7688-9

Type

conf

DOI

10.1109/FREQ.2003.1275223

Filename

1275223