Development a Risk Assessment Method of Brachyspira Hyodysenteriae in Pig Farms

Author

Yahui, Kou ; Hengan, Wang ; Yaxian, Yan ; Jian, Wang ; Peihong, Liu

Author_Institution

Sch. of Agric. & Biol., Key Lab. of Veterinary Biotechnol., Shanghai Jiao Tong Univ., Shanghai, China

fYear

2012

fDate

28-30 May 2012

Firstpage

141

Lastpage

143

Abstract

Brachyspira hyodysenteriae is a one of important pathogen of Swine dysentery. This study, we established a indirect ELISA using the expressed and purified His6-Bhlp30.7 protein as coating antigen to detect pig sera antibodies to Bhlp30.7. All the parameters of ELISA were optimized. The positive and negative sera were detected using this ELISA. And the cut-off value was determined 4.0 through comparing the results of positive and negative sera. The indirect ELISA can be used to investigate the infected level of Brachyspira hyodysenteriae in pig farm group not in individual.

Keywords

farming; microorganisms; proteins; risk management; Brachyspira Hyodysenteriae; coating antigen; expressed His6-Bhlp30.7 protein; indirect ELISA; negative sera; pathogen; pig farms; pig sera antibodies; positive sera; purified His6-Bhlp30.7 protein; risk assessment method development; swine dysentery; Biotechnology; Coatings; Diseases; Educational institutions; Proteins; Risk management; Strain; Brachyspira hyodysenteriae; Cut-off value; ELISA; Risk assessment;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macau, Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.136

Filename

6245076