EST-SNP Analysis of Grape (Vitis vinifera L.) by CAPS Marker

Author

Guo, Dalong ; Li, Meng

Author_Institution

Coll. of Forestry, Henan Univ. of Sci. & Technol., Luoyang, China

fYear

2012

fDate

28-30 May 2012

Firstpage

1449

Lastpage

1452

Abstract

CAPS marker is an efficient method to validate candidate SNP. In this study, we analyzed 42,493 grape EST sequences and 5,032 candidate SNP were identified, the amount of transversions, transitions and one base indel were 1,800, 2,896 and 336 respectively. The CAPS reaction system contained ddH₂O 5.5μL, buffer 1μL, restriction enzyme 0.5μL, PCR product 3μL. In this study, 20 grape materials were used for enzyme digestion analysis, the genotype were successfully resolved. CAPS marker is fast, low cost and efficient in grape EST-SNP analysis, and holds great promise for molecular assisted breeding or other researches of grape.

Keywords

biological techniques; enzymes; genetics; molecular biophysics; molecular configurations; CAPS marker; CAPS reaction system; EST-SNP analysis; enzyme digestion analysis; expressed sequence tag; genotype; grape; grape EST sequence; grape material; molecular assisted breeding; restriction enzyme; single nucleotide polymorphism; vitis vinifera L; Biochemistry; Bioinformatics; Educational institutions; Genomics; Materials; Pipelines; Software; CAPS; EST-SNP; grape;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.201

Filename

6245406