High-Degree Concentration of Bio-agents using Electrokinetic Manipulations

Author

Murray, Kent ; Wang, Tza-Huei ; Rebello, Keith ; Crookston, James ; Miragliotta, Joseph

Author_Institution

Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD

fYear

2006

fDate

38899

Firstpage

1

Lastpage

2

Abstract

Techniques used for the upstream collection of bio-warfare agents often result in a large, dilute sample volume composed of a wide variety of moieties. Nonetheless, current bioassays, though constantly being refined, benefit from a highly concentrated sample, leading to an enhanced signal to noise ratio and increasing the ultimate sensitivity. This report describes an electrokinetics-based sample concentration device designed to concentrate bio-agents, such as bacillus spores, from a relatively large volume (~500 mul) to be within a nanoliter volume for downstream target processing and detection. The concentrator can later be integrated with other fluidic sample processing devices and sensors for the development of a stand-alone bio-agent surveillance system

Keywords

biohazards; biological techniques; cellular biophysics; electrokinetic effects; electrophoresis; microelectrodes; microorganisms; osmosis; surveillance; bacillus spores; bio-warfare agents; bioassays; electrokinetic manipulations; electrokinetics-based sample concentration device; high-degree bio-agent concentration; stand-alone bio-agent surveillance system; Biosensors; Dielectrophoresis; Electrodes; Electrokinetics; Fluid flow; Fungi; Nanobioscience; Physics; Sensor systems; Surveillance;

fLanguage

English

Publisher

ieee

Conference_Titel

Life Science Systems and Applications Workshop, 2006. IEEE/NLM

Conference_Location

Bethesda, MD

Print_ISBN

1-4244-0277-8

Electronic_ISBN

1-4244-0278-6

Type

conf

DOI

10.1109/LSSA.2006.250373

Filename

4015844