A detection-based framework for the analysis of recycling in TIRF microscopy

Author

Chessel, A. ; Cinquin, B. ; Bardin, S. ; Boulanger, J. ; Salamero, J. ; Kervrann, C.

Author_Institution

Cell & Tissue Imaging Facility, Inst. Curie, Paris, France

fYear

2010

fDate

14-17 April 2010

Firstpage

1281

Lastpage

1284

Abstract

Endocytosis/recycling and exocytosis are mechanisms conserved through evolution allowing cells to communicate with their external medium. In order to study these dynamic processes, the present work proposes a patch-based method for detecting recycling or exocytotic events at the Plasma membrane in fast TIRF microscopy combined with the computation of normalized temporal representations of those events. Evaluation, performed on TIRF sequences showing Transferrin receptor (TfR) recycling, validates a high detection rate fully compatible with an automatic data extraction and analysis of the plasma membrane recycling process.

Keywords

bio-optics; biomedical optical imaging; biomembranes; cellular biophysics; fluorescence; image sequences; medical image processing; molecular biophysics; optical microscopy; proteins; TIRF microscopy; TIRFM sequences; automatic data extraction; cells; endocytosis; exocytosis; fluorescent trans-membrane proteins; plasma membrane; recycling; total internal reflection fluorescence microscopy; transferrin receptor; Biological system modeling; Biomembranes; Event detection; Fluorescence; Iron; Microscopy; Plasma properties; Plasma waves; Protein engineering; Recycling; TIRFM; TfR; endocytosis/recycling; event detection; patch-based;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: From Nano to Macro, 2010 IEEE International Symposium on

Conference_Location

Rotterdam

ISSN

1945-7928

Print_ISBN

978-1-4244-4125-9

Electronic_ISBN

1945-7928

Type

conf

DOI

10.1109/ISBI.2010.5490230

Filename

5490230