5-D imaging and parallel automated analysis of cellular events in living immune tissue microenvironments

The mammalian immune system consists of vital tissue microenvironments that exhibit remarkable structural complexity and dynamic cellular behaviors. It is now possible to acquire time-lapse series of multi-channel three dimensional images of multiple cell types and vasculature simultaneously, revealing dynamic events in their living tissue context. This talk will describe automated image analysis algorithms, parallel computation methods, and large-scale edit-based validation methods to detect and quantify key events such as homogeneous and heterogeneous cell-cell interactions, and methods to map events to their tissue context.