Fast laser-scanning microscopy with single- and multi-photon excitation for functional imaging of living nerve cells

Author

Iyer, V. ; Losavio, B.E. ; Saggau, P.

Volume

3

fYear

2003

fDate

17-21 Sept. 2003

Firstpage

2117

Abstract

Functional neuro-imaging is often limited by the spatio-temporal resolution of available imaging systems. The goal of this project is fast multi-site imaging of nerve cells in intact mammalian brain tissue. We will review fundamentals of fast laser-scanning microscopy employing acousto-optic beam steering and show examples of neuro-imaging applications. Specifically, we will discuss the significance and technical challenges of extending this imaging approach from single- to multi-photon excitation. We will present the current development status of a new imaging system implementing acousto-optical scanning of ultra-fast laser pulses.

Keywords

beam steering; biological tissues; biomedical optical imaging; brain; high-speed optical techniques; laser applications in medicine; optical microscopy; photoexcitation; acousto-optic beam steering; functional neuro-imaging; laser-scanning microscopy; mammalian brain tissue; multi-photon excitation; single-photon excitation; Acoustic beams; Beam steering; Biomedical optical imaging; Fluorescence; Laser beams; Laser excitation; Microscopy; Neuroimaging; Optical filters; Optical imaging;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2003. Proceedings of the 25th Annual International Conference of the IEEE

ISSN

1094-687X

Print_ISBN

0-7803-7789-3

Type

conf

DOI

10.1109/IEMBS.2003.1280156

Filename

1280156