Microfabricated templates for the electrodeposition of metallic barcodes for use in multiplexed bioassays

Author

True, Randall J. ; Taylor, Micah K. ; Chakarova, Gabriela S. ; Walton, Ian D.

Author_Institution

Nanoplex Technol., Inc., Menlo Park, CA, USA

Volume

1

fYear

2004

fDate

1-5 Sept. 2004

Firstpage

2619

Lastpage

2622

Abstract

Lithographically defined polyimide templates were prepared using microfabrication techniques. The templates consist of high aspect ratio pores that were formed by deep plasma etching. Different metals are electrodeposited into the pores, and then the template is removed to allow the metallic rods (particles) to be released into solution. The striping pattern of metals on the particles is used to optically identify the particles. Particles range in size from 0.6 μm in diameter by 7.7 μm in length to 4.2 μm by 27 μm in length. These particles have been evaluated in a multiplexed DNA hybridization assay.

Keywords

DNA; biological techniques; electrodeposition; lithography; nanoparticles; nanowires; sputter etching; 0.6 to 4.2 micron; 27 micron; 7.7 micron; DNA hybridization assay; deep plasma etching; electrodeposition; high aspect ratio pores; lithographic; metallic barcodes; microfabricated templates; microfabrication technique; microparticles; multiplexed bioassay; nanoparticles; nanowires; polyimide templates; Bioinformatics; DNA; Etching; Genomics; Nanobioscience; Nanoparticles; Niobium compounds; Plasma applications; Polyimides; Probes; DNA hybridization; ECR plasma etching; bioassays; electrodeposition; high aspect ratio; microparticles; multiplexing; nanoparticles; nanowires; polyimide;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology Society, 2004. IEMBS '04. 26th Annual International Conference of the IEEE

Conference_Location

San Francisco, CA

Print_ISBN

0-7803-8439-3

Type

conf

DOI

10.1109/IEMBS.2004.1403752

Filename

1403752