Cell-gels: hydrogels with cell-like surface modifications

A cell-mimetic object is described which consists of a pH-responsive μgel modified with a lipid membrane coating. Due to the cell-like nature of the object, it will be referred to as a “cell-gel”. It Is created by the covalent attachment of fatty acids to the surface of a microgel within a microfluidic system, forming the inner monolayer. This hydrophobic surface acts as a template for the self-assembly of the second leaflet, which spontaneously forms when the structure is exposed to phospholipid solutions. Since the lipid layer is impermeable to ions, a different pH can be maintained within the hydrogel, allowing it to remain contracted while bathed in a pH that would normally cause expansion in an unmodified hydrogel. Ultimately, biological molecules, such as receptors, will be incorporated into the bilayer. Interactions with these components can cause membrane disruption, leading to the expansion of the hydrogel from the passage of ions through the lipid layer. With further development, this object can serve as a biosensor