Development of an Integrated In Situ Analyzer for Quantitative Analysis of Microbial ATP in Aquatic Environments

Author

Fukuba, T. ; Fukuzawa, N. ; Glutz, L.S. ; Miyaji, A. ; Fujii, T.

Author_Institution

Univ. of Tokyo, Tokyo

fYear

2007

fDate

17-20 April 2007

Firstpage

240

Lastpage

244

Abstract

An integrated and miniaturized device for in situ quantitative analysis of microbial ATP was developed in this study. The device can perform extracellular ATP elimination, cell lysis and ATP quantification in a microfluidic chip. A pattern of a microchannel has been optimized to measure luminescence caused by luciferin-luciferase reaction. As a result of evaluation of the developed microfluidic device, 10 nM to 100 muM of ATP was successfully quantified. Environmental samples and bacterial culture media were also analyzed by the microfluidic device. Towards in situ calibration of the ATP quantitative analysis device, a prototype of caged ATP based online calibration component was also fabricated and evaluated.

Keywords

bioluminescence; luminescence; microfluidics; microorganisms; oceanographic equipment; oceanographic techniques; seawater; ATP quantification; aquatic environment; bacterial culture media; cell lysis; extracellular ATP elimination; integrated in situ analyzer; luciferin-luciferase reaction; luminescence; microbial ATP; microchannel; microfluidic chip; Calibration; Extracellular; Microchannel; Microfluidics; Microorganisms; Pollution measurement; Prototypes; Remotely operated vehicles; Semiconductor device measurement; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Underwater Technology and Workshop on Scientific Use of Submarine Cables and Related Technologies, 2007. Symposium on

Conference_Location

Tokyo

Print_ISBN

1-4244-1207-2

Electronic_ISBN

1-4244-1208-0

Type

conf

DOI

10.1109/UT.2007.370802

Filename

4231132