A novel magnetic tweezers for manipulation of a single DNA molecule

Author

Chiou, Chi-Han ; Tseng, Zhao-Fu ; Lee, Gwo-Bin

Author_Institution

Dept. of Eng. Sci., Nat. Cheng Kung Univ., Tainan, Taiwan

fYear

2004

fDate

2004

Firstpage

613

Lastpage

616

Abstract

We report a novel magnetic tweezers for manipulation of a single DNA molecule. The micromachined DNA manipulator can stretch and rotate a single DNA molecule using arrayed microcoils. Key platform technologies including localized DNA immobilization, microcoil fabrication and microfluidics, have been integrated to form the magnetic DNA tweezers. A single DNA molecule is specifically attached onto a magnetic bead and a gold surface and manipulated under a magnetic field generated by built-in hexagonally-aligned microcoils. A highly effective method for the construction of DNA two sticky ends is developed, which is compatible with MEMS technologies. We have successfully demonstrated the rotation of the tethered-bead DNA molecule linked to the gold pattern by circular permutation of the currents applied to the microcoils.

Keywords

DNA; biological techniques; biomolecular electronics; microfluidics; micromachining; micromanipulators; molecular biophysics; Au; DNA immobilization; MEMS technologies; deoxyribonucleic acid; gold surface; magnetic DNA tweezers; magnetic bead; magnetic field; microcoil fabrication; microfluidics; micromachined DNA manipulator; micromechanical devices; single DNA molecule; Atom optics; Atomic force microscopy; Biomedical optical imaging; Coils; Copper; DNA; Fabrication; Force measurement; Gold; Microfluidics;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 2004. 17th IEEE International Conference on. (MEMS)

Print_ISBN

0-7803-8265-X

Type

conf

DOI

10.1109/MEMS.2004.1290659

Filename

1290659