Dorsal skinfold titanium chamber for noninvasive imaging in nude mice using multiphoton and harmonic generation microscopy

Author

Li, Feng-Chieh ; Wang, Chun-Chin ; Lin, Sung-Jan ; Jee, Shiou-Hwa ; Dong, Chen-Yuan

Author_Institution

Dept. of Phys., Nat. Taiwan Univ., Taipei, Taiwan

fYear

2004

fDate

14-17 Dec. 2004

Firstpage

185

Lastpage

186

Abstract

For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.

Keywords

biomedical optical imaging; cancer; fluorescence spectroscopy; optical harmonic generation; optical microscopy; titanium; tumours; two-photon spectroscopy; Ti; animal models; diseases; dorsal skinfold titanium chamber; harmonic generation microscopy; in vivo analysis; medical-grade titanium; multiphoton microscopy; noninvasive imaging; nude mice; tumors; two-photon fluorescence microscopy; Animals; Biomedical imaging; Fluorescence; Frequency conversion; High-resolution imaging; In vivo; Information analysis; Mice; Microscopy; Titanium;

fLanguage

English

Publisher

ieee

Conference_Titel

Biophotonics, 2004. APBP 2004. The Second Asian and Pacific Rim Symposium on

Print_ISBN

0-7803-8676-0

Type

conf

DOI

10.1109/APBP.2004.1412343

Filename

1412343