Intensity correlation analysis of white light supercontinuum source for multiple wavelength laser scanning fluorescence microscopy

Author

McConnell, Gail

Author_Institution

Centre for Biophotonics, Strathclyde Univ., Glasgow

fYear

2005

fDate

17-17 June 2005

Firstpage

618

Lastpage

618

Abstract

In this paper, the white-light supercontinuum source was employed to perform sequential confocal laser scanning microscopy (CLSM) of multiple-labelled guinea pig detrusor. The broad-bandwith output was easily manipulated using a simple grating structure to provide the wavelength ranges closely matching the absorption profiles of the desired fluorophores. To ascertain the effect of the noise fluctuations on the resultant CLSM image, intensity correlations of both the Ti:sapphire platform laser and the white-light supercontinuum are presented

Keywords

fluorescence; laser applications in medicine; laser noise; optical microscopy; sapphire; solid lasers; supercontinuum generation; titanium; Al₂O₃:Ti; Ti:sapphire platform laser; absorption profile; fluorophore; grating structure; intensity correlation; intensity correlation analysis; multiple wavelength laser scanning fluorescence microscopy; multiple-labelled guinea pig detrusor; noise fluctuation; sequential confocal laser scanning microscopy; white light supercontinuum source; Fiber lasers; Fluctuations; Fluorescence; Laser noise; Laser theory; Microscopy; Photonic crystal fibers; Pixel; Pump lasers; Supercontinuum generation;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Europe, 2005. CLEO/Europe. 2005 Conference on

Conference_Location

Munich

Print_ISBN

0-7803-8974-3

Type

conf

DOI

10.1109/CLEOE.2005.1568394

Filename

1568394