Biochemical IC chip toward cell free DNA protein synthesis

Author

Ikuta, Koji ; Maruo, Shoji ; Fukaya, Yuichiro ; Fujisawa, Tetsuya

Author_Institution

Dept. of Micro Syst. Eng., Nagoya Univ., Japan

fYear

1998

fDate

25-29 Jan 1998

Firstpage

131

Lastpage

136

Abstract

The several type of biochemical IC chips with photo detective micro reactor has been developed. The real time monitoring and controlling cell free synthesis of protein was demonstrated successfully. The photo protein of firefly so called Luciferase was synthesized. The biochemical luminescence was monitored by built-in photo diode in the micro reactor to transfer reaction status into voltage signal. Feedback control synthetic reaction was verified experimentally. The results achieved here should be key technology for future DNA and RNA synthesis without biological cell

Keywords

DNA; biocontrol; biological techniques; chemical technology; elemental semiconductors; feedback; integrated circuit technology; micromechanical devices; proteins; silicon; Luciferase; RNA synthesis; Si; biochemical IC chip; biochemical luminescence; built-in photo diode; cell free DNA protein synthesis; feedback control synthetic reaction; micro reactor; photo detective micro reactor; protein; reaction status; real time monitoring; voltage signal; DNA; Diodes; Feedback control; Inductors; Integrated circuit synthesis; Luminescence; Monitoring; Proteins; Signal synthesis; Voltage;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro Electro Mechanical Systems, 1998. MEMS 98. Proceedings., The Eleventh Annual International Workshop on

Conference_Location

Heidelberg

ISSN

1084-6999

Print_ISBN

0-7803-4412-X

Type

conf

DOI

10.1109/MEMSYS.1998.659742

Filename

659742