Impedance spectroscopy studies of electroporated human breast cancer cells

Author

Xiao, Funian ; Otto, Kevin ; Camarillo, Ignacio ; Sundararajan, Raji

Author_Institution

Purdue Univ., West Lafayette, IN, USA

fYear

2009

fDate

18-21 Oct. 2009

Firstpage

470

Lastpage

473

Abstract

The passive, electrical impedance characteristics of electroporated, MCF-7 human breast cancer is described in this paper. For this purpose, an Autolab impedance spectroscope was used. The electroporation protocol consisted of 200 V/cm, 40 ms, and 1200 V/cm, 200 Â¿s pulses. The impedance characteristics of these cells displayed a negative fractal power law as expected for dielectrics. They also exhibited single dispersion Cole-Cole model. Our experiments demonstrated that the various electroporation treatment parameters result in the different curvatures of Cole-Cole model. This phenomenon proves that the effects of electroporation drug delivery to cells could be controlled in real time by monitoring impedance characteristics.

Keywords

biochemistry; bioelectric phenomena; biological organs; cancer; cellular transport; drug delivery systems; drugs; gynaecology; Autolab impedance spectroscope; electrical impedance characteristics; electroporated human breast cancer cells; electroporation drug delivery; electroporation protocol; electroporation treatment parameters; impedance spectroscopy; negative fractal power law; real time monitoring; single dispersion Cole-Cole model; time 200 mus; time 40 ms; Biological materials; Bovine; Breast cancer; Cells (biology); Dielectrics; Drugs; Electrochemical impedance spectroscopy; Frequency; Humans; Medical treatment;

fLanguage

English

Publisher

ieee

Conference_Titel

Electrical Insulation and Dielectric Phenomena, 2009. CEIDP '09. IEEE Conference on

Conference_Location

Virginia Beach, VA

ISSN

0084-9162

Print_ISBN

978-1-4244-4557-8

Electronic_ISBN

0084-9162

Type

conf

DOI

10.1109/CEIDP.2009.5377789

Filename

5377789